Study Question: Does micronutrient and probiotic supplementation in oocyte donors improve fertilization rates, blastocyst development, and pregnancy outcomes?

Summary Answer: Supplementation did not improve fertilization or blastocyst development rates, but significantly improved pregnancy outcomes in recipients using vitrified oocytes.

What is Known Already:

• Micronutrients may improve IVF outcomes, but evidence on oocyte quality in normo-responders is lacking.
• OvoVid double blind, randomized, controlled trial Phase 1 showed supplementation increased oocyte yield, maturity, and follicular response in donors.

Study Design, Size, Duration:

• Design: Multicentric, double-blind, randomized controlled trial
• Duration: 2 years
• Participants: 130 oocyte donors and 107 recipients
• Study Design & Duration: One tablet daily ≥30 days before and during COH containing myo-inositol, d-chiro-inositol (40:1), folic acid, melatonin, coenzyme Q10, vitamin D3, B6, B12, E, iron, zinc, Lactobacillus gasseri and crispatus. v/s placebo.
• Outcomes:
  1. Primary: Fertilization and blastocyst rates
  2. Secondary: Pregnancy rates, oocyte survival post-thaw
• Analysis: Fisher’s exact test, Mann-Whitney U test (p<0.05)

Main Results:

1. Fertilization rate: No significant difference (76.7% supplement vs 78% placebo, p>0.05)
2. Blastulation: No significant difference (43.4% vs 45.4%, p=0.61)
3. Pregnancy rate overall: Not significant (44.4% vs 68.2%, p=0.07)
4. Vitrified oocytes: Pregnancy rate significantly higher in supplement group (50% vs 0%, p=0.02)
5. Fresh oocytes: No significant difference

Limitations: Small subgroup sample sizes and lack of long-term follow-up limit generalizability. Further studies are needed.

Wider Implications: While supplementation did not improve fertilization or blastulation rates, it may enhance pregnancy outcomes with vitrified oocytes, suggesting potential benefits in optimizing oocyte competence for vitrification protocols.

ESHRE 2025, June 29 - July 2, Paris